文档介绍：Purification and Identification of Acidic Proteins
in Bovine Colostrum
1
Bing HAN ; Ming DU2, *; Weili XU2; Haibo GAO2
College of Food Engineering, College of Food Science and Engineering,
Harbin University merce, Harbin Institute of Technology,
Harbin 150028, China Harbin 150090, China
Email: hb790118@ duming2006@
Abstract—Colostrum contains higher concentrations of Therefore, the objective of this study was to purify and
immune-enhancing nutrients ponents than mature identify positions of BCAP.
milk and its bioactivity has received widespread attention.
In the present study, the protein fractions of bovine
colostrum whey were isolated and purified by membrane
filtration, series of chromatography on DEAE52 ion II. MATERIALS AND METHODS
exchange column, gel filtration column and Econo–pac Q A. Chemicals and materials
column. Four kinds of dominant peptides in BCAP,
lactoferrin, osteopontin, insulin-like growth factor-2 and Sephadex G-100 was purchased from Pharmacia Co.
epidermal growth factor, were purified and identified. (Amersham Bioscienses, Uppsala, Sweden). Econo–pac
These ponents are devoted for the beneficial Q column (× cm) was purchased from Bio-RAD
bioactivities of colostrum whey on preventing or delaying Co. (Bio-RAD Co. in Beijing, China). All other
osteoporosis. chemicals were of analytical grade.
Keyw