文档介绍：Preliminary Study on the Factors on Callus Induction
of Phalaenopsis
Shuguo Fan 1,2, Qibin Zhang 1, Lu Qiu 1,2*, Guoshu Li 1,2, Haiyan Yang 1, Ming Jiang 1
1Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong 675000;2Institute of Bio-resources Research
and Utilization in Central Yunnan,Chuxiong 675000
Abstract-The explants (root tip, shoot tip, stem segments and leaf Tissue culture is an effective pathway for Phalaenopsis
segments) from Phalaenopsis hybrid were used in this rapid multiplication, and callus induction is the first step for
experiment. The effects of NAA, 6-BA, AC, CM and above plant regeneration, which is of significant theoretical value for
explants on callus induction on KC medium were investigated by callus induction of Phalaenopsis. Cytokinin is very important
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using orthogonal design (L16(5 )). The results indicated that the for plant somatic embryogenesis, which can play this role
optimum medium for callus induction is 6-BA mg/L+ NAA alone, but in some plants, somatic embryogenesis can only be
mg/+CM 100 ml/L+AC mg/L, and stem segment was used induced by both cytokinin and auxin (3). Cytokinin plays a
as explant, and the highest induction rate was 52 % as well. The key role in PLB induction and cell multiplication in orchid
statistical results by showed that the hormones have