文档介绍：Lipase-catalysed Synthesis of Ferulyl Oleins and
Evaluation of Their Radical Scavenging Activity
Lin-lin Chen, Jia-ying Xin
Key Laboratory for Food Science & Engineering,
Harbin University merce, Hrbcu
Harbin, People’s Republic of China
linclear1207@
Abstract—The enzymatic transesterification of ethyl ferulate
with triolein was investigated. Ferulyl oleins were synthesized by O CH
O 3
immobilized lipase from C. antarctica (Novozym 435) anic
phase, solvent-free system, and glycerol introduced system. The H2C O C CH CH OH
transesterification reactions in those three different solvent
systems pared. The yield anic medium was the HC O R1
lowest and the selectivity to FMO was higher in glycerol
introduced system than the other two system. 4Å molecular sieve H2C O R2
was selected to control the reaction water content. The yield of 1 R1= (CH2)7 CH CH (CH2)7 CH3, R2=H
ferulyl oleins increased with increasing the amount of molecular
(CH ) CH CH (CH ) CH
sieve to 20%. The radical scavenging activity of the products 2 R1=R2= 2 7 2 7 3
were evaluated using DPPH. Ferulyl monoolein was found to
possess higher radical scavenging activity than one of the Fig. 1. Chemical structures of strutures of ferulyl oleins. 1. FMO and 2. FDO.
substrates, ethyl ferulate. The time to reach steady state of ferulyl antioxidant activity than the acid itself