文档介绍：Screening a novel high effective phenol degradation
strain and its characters
Jifeng Guo
School of Environmental Science and Engineering, Chang’an University, 710064
Xi’an, China
E-mail: gjf01398@
Abstract—A bacterial strain was obtained through the screening
experiments of position of phenol, which had high II. MATERIALS AND METHODS
degradability for phenol and was named P-1. The gene sequence
obtained from the DNA extraction and polymerase chain reaction A. anisms Origin
(PCR) amplification pared with NCBI Genbank of Most of samples came from the sludge of sewer in vicinity
., and the strain was preliminary identified to be of phenol factory [1-4], and the high effective strains had been
us sp. The optimum growth conditions were pH , 30 got. But there was no unaware that if they can be used in the
℃, 150r/min, 30-50mL/250mL (liquid volumes/flask volumes). real activated sludge process well. So we got the sample from
When the phenol concentration was above 800mg/L, P-1’s phenol the activated sludge in A/O tank of chemical factory
degradation ratio decreased with the increase of the phenol wastewater station in Xi'an.
concentration. The strain had strong adaptability to the
environmental conditions, which showed it was a high effective
phenol degradation strain and had better application prospects B. Culture Medium
in wastew