文档介绍：Effect of Cultivating Conditions on γ-Aminobutyric
Acid Production by Lactobacillus brevis
Weiqing CHEN Xiaofeng WANG
College of Biological and Environmental Engineering College of Pharmaceutical Science
Zhejiang Shuren University Zhejiang University of Technology
Hangzhou, China Hangzhou, China
cwq135790@
Abstract: To investigate the optimum culture conditions of γ-
aminobutyric acid (GABA) production by a -W11 II. MATERIALS AND METHODS
identified as Lactobacillus brevis in submerged fermentation,
certain fermentation parameters involving inoculum volume of A. anism and culture medium
seeds, incubation temperature, initial pH value, period of -W11 was isolated from the Chinese traditional
cultivation and glutamate concentration in medium were yogurt and identified as Lactobacillus brevis according to
investigated separately. The optimal cultivating conditions of Bergey's Manual of Systematic Bacteriology. 60Co γ-ray was
GABA production in submerged fermentation were MRS used to mutate the strain, and obtained a positive mutant strain
medium with 5% monosodium glutamate (MSG), 32°C with relative high GAD activity. It was reserved in the
incubation temperature, 5% inoculum volume, initial pH of biotechnological laboratory of Zhejiang Shuren University.
medium, and 60 hours cultivation period. Under the optimized
cultivation co