文档介绍：Detection of biomolecular binding by
Fourier-Transform SPR
Layne D. Williams, Renny E. Fernandez, Farouk Azizi, Tridib Ghosh and Carlos H. Mastrangelo
University of Utah
Department of Electrical puter Engineering
Salt Lake City, UT, USA
layne.******@
Abstract— Surface plasmon resonance (SPR) is a widely used
label-free detection technique that has many applications in drug
discovery, ics, systems biology and food science.
The SPR technique measures the dynamics of a biomolecular
interaction at a surface, yielding ic association and
dissociation constants. Present SPR systems measure the step
response of the interaction in time domain hence are subject to
time-varying noise disturbances and drifts that limit the
minimum-detectable mass changes. This paper presents a new
synchronous SPR technique that measures the biomolecular
interaction not in time domain, but in frequency domain with a Fig. 1: Diagram of chemical reaction and sensor response to single
high degree of rejection to uncorrelated spurious signals. The association and dissociation steps.
new technique was implemented using a PDMS microfluidic
chemical signal modulator chip connected to a set of on-chip surface concentration of the ligand-plex [2]. For a
functionalized Au SPR sensing sites. Preliminary experimental
sudden step change in the ligate con