文档介绍：Detection and Identification of Enterohemorrhagic
Escherichia coli O157:H7 using Agilent 2100
Bioanalyzer
FENG Jian1, BAI Yan-hong1, WANG Yun-long2, JING Jian-zhou1*
(1College of Food and Biological Engineering, Zheng Zhou University Of Light Industry, Zhengzhou Henan, 450002, China;
2 Henan Bioengineering Technology Research Center, Zhengzhou 450002, China
3 E-mail address: ******@zzuli.)
Abstract-To detect and identify of Enterohemorrhagic species or serogroup level and do not directly characterize
Escherichia coli O157:H7, the gene rfbE of O157:H7 was detected virulence factors.
as the aim gene and the Agilent 2100 bioanalyzer was used as an
analysis tool, which pared with the conventional agarose The Agilent 2100 Bioanalyzer provides a robust platform
gel electrophoresis. Finally, it was known that the rfbE gene was or the quantification and high resolution of DNA fragments
essful in detection of O157:H7. And the bioanalyzer was as via electrophoresis in microfluidics chips [5]. This platform has
10 times sensitive as the agrouse gel electrophoresis; at the same been utilized in various studies, primarily with the goal of
time it could give the accurate size, whose error rate was less
eplacing or improving existing techniques, such as RFLP [6, 7],
than 5%. The conc and molarity of the different Cell
or attaining the visu