文档介绍：Molecular Cloning of the cDNA of LPL on Goose
and the ic relationship in the lipase
superfamily
Hengyong Xu , Yapan Song, Chunchun Han, Yan Wang, Liang Li, Feng Xu, Qing Zhu, Jiwen Wang*
College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan, 625014, P. R. China.
Abstract—Lipoprotein lipase (LPL) of Tianfu meat goose regulate its tissue-specific activity [8]. For example, LPL
was cloned and sequenced using a RT-PCR approach. The expression is increased during lactation in the mammary gland
[19, 20]
nucleotide sequence covered 468 bp with an open reading and through development in adipose tissue, skeletal
[21]
frame of 155 amino acids. Alignment analysis indicated muscle and heart .
that the nucleotide sequences are highly conserved to other Goose represents a good model for studying the lipid
species studied including chicken, domestic guinea pig, metabolism, because it uses lipid as energy preferentially,
house mouse, Norway rat, cattle, cow and goat, and the which is different from mammals using carbohydrate for
homologies are %, %, %, %, energy usually. Moreover, the fatty liver in waterfowl was
%, % and %, respectively. Topology depends on the peripheral activity of LPL. So, in this study,
prediction showed goose LPL including two N-linked our aim was to clone the cD