文档介绍：8个绣球品种耐热性综合评价与耐热指标筛选     凌瑞 戴中武 代晓雨 吴春梅 翟俊文 郑泽新 吴沙沙 摘  要：夏季高温已成为制约绣球引种与园林應用的首要限制因子，为构建绣球品种耐热性评价体系并筛选耐热品种，本研究以8个绣球品种为材料，对其高温半致死温度（HLT50）及其高温胁迫下热害指数（HII）进行观测，通过测定44 ℃/33 ℃（昼/夜）高温胁迫6 d后的14项生理生化指标，采用多元统计分析方法对其耐热性进行综合评价。结果表明：通过主成分分析法将原有14项指标转化为4个彼此独立的综合指标，利用隶属函数法得到各品种的综合评价值（D值），通过聚类分析将8个绣球品种分为3个类群，耐热型包括‘花手鞠与‘头花;中等耐热型包括‘爱莎‘含羞叶‘小町和‘纱织小姐;不耐热型包括‘银边与‘灵感。在此基础上采用逐步回归法建立了以游离脯氨酸（Pro）含量、叶绿素（Chla+Chlb）含量、类胡萝卜素（Car）含量、叶绿素a/b值（Chla/Chlb）、超氧化物歧化酶（SOD）活性、相对含水量（RWC）与可溶性糖（SS）含量7个指标为变量的耐热性评价模型。此外，HLT50与HII也可作为鉴定绣球品种耐热性的可靠方法。本研究可为绣球品种在南方城乡园林中的应用推广以及耐热型绣球品种选育提供理论依据。 关键词：绣球品种;耐热性;生理生化;隶属函数法;综合评价体系 Abstract： High temperature is one of the main limiting factors for the introduction and landscape application of Hydrangea in summer. In order to construct the heat resistance evaluation system of Hydrangea cultivars and select heat resistant cultivars， eight Hydrangea cultivars were used to observe the high temperature semi-lethal temperature （HLT50） and the heat injury indexes （HII） under high temperature stress， and 14 physiological and biochemical indexes were measured after six days of high temperature stress at 44 ℃/33 ℃ ， after which heat resistance was evaluated by multivariate statistical analysis method. The original 14 indexes were transformed into four independent comprehensive indexes by the principal component analysis， and the comprehensive evaluation value （D value） of each variety was obtained by the subordinate function method. According to D value， the eight Hydrangea cultivars were divided into three groups. The moderate heat resistance cultivars included ‘Essar ‘Elbtal ‘Komachi and ‘Shakespeare. The heat sensitive cultivars included ‘Tricolor and ‘Inspiration. The heat resistance cultivars included ‘Stockings and ‘Corsage. On the basis of D value， the stepwise regression method was used to set up the variable heat resistance evaluation model with the content of proline （Pro）， chlorophyll （Chla+Chlb）， carotenoid （Car）， chlorophyll a/b value （Chla/Chlb），