文档介绍：◎药用植物化学与中药有效成分分析研讨会论文集(下) 复方QCLU提高小鼠免疫功能研究王利,林锦骠,史媛,张秋玉,沈佰华,李宁丽+ 上海市免疫学研究所上海交通大学医学院上海200025 摘要:目的:通过研究中药复方QCLU提高小鼠免疫功能的机理与途径,探索该制剂对于治疗免疫低下和免疫缺陷的临床潜能。方法:制备小鼠免疫低下模型。采用口服灌胃的方法给小鼠口服复方QCLU。于灌胃结束后取小鼠外周血和胸腺细胞悬液采用特异性抗体染色并经流式细胞仪染色观测小鼠上述细胞数量变化。处死小鼠后取小鼠脾脏单个核细胞加抗CD3和CD28刺激并经同位素掺入试验观察小鼠淋巴细胞增殖能力。结果:1、复方QCLU具有明显上调小鼠外周血T细胞和CD4+T细胞数量的作用:给免疫抑制模型小鼠口服复方QCLU后可有效增加小鼠T细胞及CD4+T细胞数量:非服用组T细胞从12±3%,服用组T细胞为28±4%,两组相比具有显著差异(p<);而CD4+T细胞从5±2%(非服用组)增加至22±3%(服用组),两组相比具有显著性统计学差异(p<)、胸腺内CD4+CD8+T细胞增多。同时服用复方QCLU小鼠的T细胞对抗CD3和CD28刺激的增殖反应明显增高(p<)。结论:复方QCL具有明显上调小鼠T细胞和CD4+T细胞,其作用可能是通过刺激胸腺内T细胞成熟有关。关键词:获得性免疫缺陷综合症,复方QCLU口服液, CD4+T细胞,增殖反应 Abstract:Smdy onmechanisms ofQCLU,an oralsolution,enhalice immune response mmice Sheng Baihua,Wang Li,Shen Tianwei,SHeng Huiming,ZhangQiuyu,SJli Yuan,Lill Jingpiao,Li Ningli, Shanghai InstituteofImmunology,Shanghai Jiaotong University School ofMedicine,Shanghai,200025 Acquired immunodeficiency syndrome(called ADS)is akind ofserious infectious disease which Call cause death and spread rapidly SO far,there arehaven’tany substantial progress inthe prevention and cure thisrepo疋we report thatQCLU,plex extracts from Chinese herbs, showed enhance immune response in vivoand showed up—regulation T ceilsinwhich CD4—-T cellsincreased obviously than CD8+T showed increaSe Tcells proliferation stimulatedby anti—CD3