文档介绍：不同剂型夏桑菊颗粒 HPLC 指纹图谱及其模式识别分析[ 摘要] 建立夏桑菊有糖和无糖颗粒的 HPLC 指纹图谱, 为其鉴别与有效控制质量提供参考。采用高效液相法采集 20 批无糖型和 34 批有糖型夏桑菊颗粒指纹图谱, 通过模式识别方法( 主成分分析, 正交最小二乘法判别分析) 分类并筛选其主要差异组分; 通过对照品对照的方法鉴定其主要的组分。成功建立夏桑菊有糖和无糖颗粒指纹图谱; 主成分分析不能完全分类 2 种颗粒,而正交最小二乘法判别分析可明显的分为 2 类; 2 种颗粒之间差异最大的组分主要有 6个, 其中 3 个分别为异迷迭香酸苷、木犀草苷和蒙花苷。该研究建立的模式识别方法有助于夏桑菊颗粒整体质量控制,同时为其质量评价提供一种有效手段。[ 关键词]HPLC 指纹图谱;模式识别;夏桑菊颗粒;主成分分析;正交最小二乘法判别分析[Abstract]To establish the fingerprints of Xiasangju granules ( with sugar and non-sugar forms ) by HPLC , and provide reference for their identification and effective quality control. High performance liquid chromatography ( HPLC ) method was used to collect the fingerprints of 20 batches of non-sugar Xiasangju granules and 34 batches of sugar type Xiasangju granules. Their main ponents were classified and screened by mode identification methods ( ponent analysis , PCA , and orthogonal partial least squares discriminate analysis , OPLS-DA ). The ponents were identified paring with reference standards. The fingerprints of Xiasangju granules ( sugar type and non-sugar type ) were established. PCA could not fully classify the two types of granules , while OPLS-DA could obviously classify these two different types of Xiasangju granules. ponents showed greatest difference between two types of granules , including salviaflaside , luteoloside and linarin. The developed mode identification method is helpful to control th