文档介绍:Physiology of bacteria
Demonstration
Biologic factors
Disk diffusion susceptibility test
Specific lysis test of bacteriophage
Biochemical reaction
Lactose fermentation test
Hydrogen sulfide test
Urease test
Operation
IMViC tests(Continued)
(E. coli、 E. aerogenes )
Effection of
Ultraviolet radiation
(E. coli、 B. subtilis )
Streak plate method (Continued)
(mixed bacteria)
Video
History of microbiology
Inhibition zone抑菌圈
Disk diffusion susceptibility test
PEN Penicillin青霉素
STR Streptomycin链霉素
GEN Gentamicin庆大霉素
CEC Cefaclor头孢克洛
PEN
GEN
STR
CEC
1
2
3
broth
Bacteriophage of
Specific lysis test of bacteriophage
Plaque
噬菌斑
Carbohydrate Fermentation Tests 糖发酵试验
carbohydrate HCOOH CO2+H2
H2S test
Urease Test
尿素酶试验:
Sulfur-containing amino acid
( Methionine ,Cysteine, Cystine)
H2S
Pb2+
Fe2+
PbS
FeS
(black)
urea尿素
ammonia+  carbon dioxide
氨 二氧化碳
Urease
E. coli
B. subtilis
班级、姓名/学号、 B,subtilis
uv30’
incubator
Effection of Ultraviolet radiation
Indol test 吲哚试验
Tryptophan色氨酸
↓
Indole吲哚↑
Kovac's reagent ( paradimethylaminobenzaldehyde
对二***氨基苯甲醛)
pink/red layer forming(玫瑰吲哚)
- +
Methyl red test ***红试验
- +
(+)
Glucose
Pyruvate
***酸
Glucose
pyruvate
acetylmethyl carbinol
乙酰***甲醇
(-)
pH<
pH>
VP(Voges-Proskauer)test
(+)
(-)
alpha-naphthol萘酚
Glucose
Pyruvate
***酸
Glucose
pyruvate
acetylmethyl carbinol
乙酰***甲醇
Diacetyl
二乙酰
KOH
Arginine
(Guanidyl胍基)
red compound