文档介绍:
利用不同分子标记分析^p 烟草种质资多态性
【摘要】:^p [目的]研究烟草种质资的遗传多样性及亲缘关系。[方法]利用SSR、EST-SSR和InDel这3种分子标记对53份不同类型的烟草材料进行区别与鉴定。[结果]53份烟草材料可分为2大类群,2个亚类;与EST-SSR和SSR分子标记相比,InDel分子标记以其扩增谱带少、特异性强,易于识别和统计,更适合用于烟草种质资遗传多样性分析^p 与研究。[结论]该研究能够较好地揭示烟草栽培品种类型间的遗传多样性与亲缘关系,可为烟草种质鉴定和遗传连锁图谱构建提供科学依据。
【关键词】:^p 烟草;分子标记;遗传多样性
中图分类号 S188 文献标识码 A 文章编号 0517-6611(20__)29-0139-05
Analysis of Geic Diversity in Tobacco Germplasms (Nicotiana tabacum L.) Using Different Molecular Markers
FENG Ji, CHENG Ling, SUN Guangwei, CAI Changchun_
et al
(Tobacco Research Institute of Hubei Province, Wuhan, Hubei 430030)
Abstract [Objective]To research geic diversity and relationship in tobacco germplasms.[Method] 53 tobacaterials were distinguished and identified by SSR, ESTSSR and InDel.
[Result]53 tobacaterials could be classified into two groups and two with the ESTSSR and SSR molecular marker, it was less lification band, strong specificity, easy identification and statistics for InDel marker.
Therefore, InDel molecular marker was more suitable for geic diversity analysis and research of tobacco germplasm.
[Conclusion]The study is useful in revealing the geic diversity and relationships among tobacaterials, and providing a scientific basis for the germplasm identification and geic linkage ming.
Key words Tobacco;Molecular markers;Geic diversity
基金項目 中国烟草总公司湖北省公司重点项