文档介绍：摘要本试验以槲蕨(Drynaria roosiiNakaike)孢子为外植体材料,利用植物组织培养的方法,通过正交实验设计及统计分析方法,研究外植体灭菌时间、孢子采样时间、基本培养基、植物生长调节剂、移栽基质配方等因素对孢子离体快繁的影响,筛选出各培养阶段的适宜培养条件,进行槲蕨孢子离体培养快繁技术研究, 以期建立槲蕨组织培养快速繁殖技术体系,结果表明: l、不同的灭菌时间影响到孢子繁殖的污染问题,槲蕨孢子初代培养,用70% 酒精过滤消毒30s+0。1%升***消毒lmin灭菌效果最好,无菌外植体获得率为 %;最佳基本培养基为MS培养基。 2、槲蕨孢子无菌培养最佳采样时间在5月中下旬,那时孢子成熟最好最适合作为接种外植体,此时孢子较成熟,灭菌时间也较好把握,孢子萌发率高达80%, 且生长状况良好,有利于进行无菌培养。 ,6-BA是孢子体增殖培养的主要影响因子。MS+6-++NAA ,。 + IBA,生根率达97%,平均生根数 ,。生长素是生根培养的必需成分,活性炭对生根无显著影响。 :槲蕨组培苗移栽以疏松且保水的圃地表土为基质最好,且生长状况良好,成活率达98%。关键词:槲蕨,孢子繁殖,组织培养,愈伤组织,快速繁殖万方数据 Abstract This Paper hasDrynaria roosiiNakaike as thematerial tousing themethod of plant tissueculturebyorthogonal experimental design and statisticalanalysis methods, research the effects to spores invitro propagation by the factor of explants sterilizationtime、sampling time ofspore,basic medium,plant growth regulator? prudentialtransplanting plant massformula。Through these methods Can beselected the suitable cultureconditions foreach stage totheresearch of invitroculture technique on Drynaria roosiiNakaike which inorder toestablish Drynaria roosii Nakaike tissuecultureandrapid propagation technology resultsshows: timepollution problems affecting reproductive spores, Drynaria roosiiNakaike sporesprimaryculture,The beststerilizationeffect:Through treming with70%alcohol filterfor30 seconds、%mercuric chloride for 60 seconds;The rate ofobtaining sterileexplants %:The bestbasic culture medium iSMS。 bestsampling time isinlateofMay,because ofthetime when thespore mature bestsuitableasexplants,and thespores are more mature, Sterilization time rate ofspore germination is80%,and growth ingood condition, isconducive tosterileculture. growth regulators on the Spore propagation subculture andproliferation has great influence,6-BA isthe ma