文档介绍：(制)、荜茇、诃子三味药材组成的丸剂,是蒙医常用成药。本文对其质量评价方法进行研究。采用薄层色谱法(采用硅胶GF254板,在紫外灯254nm下观察)、没食子酸进行了定性鉴别。.***(v:v:v =7:2:1)为展开剂,;以***(v:v:v =7:2:1)为展开剂,。分别采用冷浸法、***、中***和次***的提取效果进行了考察,结果显示超声提取法最好。研究了超声提取法中溶剂的影响。确定最佳提取工艺为: 10%氨水润湿后,用***作为溶剂超声提取。采用反相高效液相色谱法(Hypersil ODS色谱柱),:草乌(制)中的3种乌头类生物碱(鸟头碱、中***和次***)、荜茇中的胡椒碱及诃子中的没食子酸5种成分进行了定量分析:.******(v:v:v:v=60:40:2:)为流动相,在254nm 进行检测,次***、***和中***~·mL“范围内峰面积与浓度呈线性关系,其回归方程分别为A,; 106C+×104、A2 =×10%+×104、Aj=×,、,%、%%, %、%%;(v:v=77:23)为流动相,在姿堕垫型查堂堡主兰竺笙兰塑塞 343nm处进行检测,胡椒碱在12-84I_tg·mLl范围内的峰面积与浓度呈良好线性关系,其回归方程为4;,r=,%,%;-L‘1磷酸水溶液(V:V;5:95) 为流动相,在280rim处进行检测,没食子酸在80--·mL“范围内峰面积与浓度呈良好线性关系,其回归方程为A=,,= ,%,%。研究结果显示:本研究建立的分析方法灵敏度高,重现性好,为那如一3丸质量评价提供了依据。关键词:,制草乌,荜茇,诃子,质量评价 2 鲨塑堑型奎堂堡主兰堕垒奎竺!坐!! STUDIES ON QUALITY ASSESSMENT METHODS OFMONGOLIAN RECIPE NARU-3 ABSTR ACT The extraction process and quality assessment oftheMongolian recipe pill,which posed of Radix Aconiti Kusnezoffii Preparata, Fructus Piperis Longi and Fructus Chebulae were studied inthisthesis. Piperineand GallicacidinNaru-3 pill were identifiedby TLC withthe ponent dotsand verygood was identifiedusing silicagel GF2“、析t11benzene—ethylacetate-acetone(v:v:v=7:2:1)as developer and observed at254nm using Piperine Was 0,40. Gallic acid WaS identified using silicagel GF254 with chloroform-ethyl acetate-formic acid(v:v:v=7:2:I)as developer and observed at254nm using . A reliableextraction method ponents from thedrug Naru·3 was set up and purification ofthe extract for the quantitative determination of the aconitine alkaloids:aconitine,hypaconitine, mesacon