文档介绍：浙江大学硕士学位论文红肉脐橙和普通脐橙果实类胡萝卜素合成基因的差异表达姓名:金蓉申请学位级别:硕士专业:果树学指导教师:徐昌杰 20070501 缩略词表英文缩写英文全称 AMP ampiciUin BCH carotene 13-S cDNA CS C丑气B DEPC 中文全称氮苄青霉紊胡萝h紊8环羟化酶 capsanthin/capsorubin synthase 辣椒红,plementary DNA citratesynthase cetyltrimethyl ammonium bromide diethylpyrocarbonate 互补DNA 柠檬酸合成酶十六烷基三***溴化铵焦碳酸二乙酯 DM_D巾P dimethylallyl diphosphate 3,3.=***丙烯基焦磷酸 DNase I deoxy Ribonuclease I 脱氧核糖核酸酶I DⅫ DXR DXS EB ECH EDl’A GGPP GGPS HDS HMBPP 1-deoxy-D-xylulose 5-phosphate 1-deoxy-D-xylulose 5-phosphate reductoi$omerase 1·脱氧木*** 1一脱氧木***糖- 1-deo)cy·-phosphate synthase 1-脱氧木*** ethidium bromide 溴化乙锭 carotenes-ring hydroxylase 胡萝h素￡环羟化酶乙二***四乙酸橇牛几基犍牛几基焦磷酸 geranylgeranyl pyrophosphate synthase栊牛儿基糖牛儿基焦磷酸合成酶(E)_4-hydroxy-3-methylbut-2-enyl diphosphate synthase (E)4hydroxy-3-methylbut-2-enyl diphosphate m (E)4羟基一3-甲丁-(EM—羟基一3-甲丁-2-烯基二磷酸英文缩写英文全称 lDI IDS IPP LB LCY-b MCK MCS MCT isopentenyl pyrophosphate dimethylallyl diphosphate isomerase isopentenyl pyrophosphate dimethylallyl diphosphate synthase 中文全称异戊烯焦磷酸,3,3-二***丙烯基焦磷酸异构酶异戊烯焦磷酸,3,3-二***丙烯基焦磷酸合成酶 isopentenyl pyrophosphak} 异戊烯焦磷酸 Lu晒Bertanl medium lycopenep-cyclase Iycopene s-cyclase LB培养基番茄红素B环化酶番茄红寨e环化酶 2-C’methyl-d-eqlthdtol4-phosphate kinase 2-C.***一弘赤藻糖醇4磷酸激酶 2-C-methyl-d-erythdtol-2 4-cyclodiphosphate 2-C.***.-2, synthase 合成酶 2-C-methyl-d-erythritol4-phosphate cytidyl 2-C一*** transferase 移酶 MEP 2-C-methyI-D-erythfitol4-phosphate messenger RNA NXS neoxanthin synthase PCR polymerase chain reaction PDS ph”oene desaturase PSY phytoene synthase RHasc A ribonudease A Rpm VDE ZDS ZEP revolutions per minute violaxanthin de-epoxidase ‘-carotene desaturase zeaxanthin epoxidase Ⅳ 2-C一***一D· 娥t贷铵堇莱黄素脱环氧酶÷胡萝}素脱饱和酶玉米黄素环氧酶摘要类胡萝卜素是柑桔果实重要色素,其组分与含量影响果实色泽和保健功效。柑桔类胡萝h素分子研究多集中于合成途径的下游部分。对上游途径少有涉及。红肉脐橙积累高含量的类胡萝h素,但其分子机制尚不清楚。本研究从甜橙中分离了卜脱氧木***糖一5一磷酸合成酶(脚回和卜脱氧木***糖-5-磷酸还原异构酶(删基因片段,并对红肉脐橙和普通脐橙中7种类胡萝h素合成基因的表达差异进行了分析。以甜橙成熟果实的果皮cDNA为模板,应用普通P