文档介绍：摘要目的:了解射精管梗阻对大鼠睾丸生精功能的影响及五子衍宗丸早期干预效果。阐明射精管口梗阻(EDO)不育症的睾丸生精功能损伤机制,并且可以为五子衍宗丸治疗EDO不育症提供基础实验依据,为EDO不育症提供一条崭新的治疗思路,改变目前EDO不育患者仅仅依赖经尿道射精管口切开术(TURED)的单一治疗模式、大幅度提高该类患者的自然生育率,从而可节约高昂的人工辅助生殖费用并可避免由此带来的一些伦理纠纷问题。方法:第一步:[体重(300±20)g]选育并随机分组,,检测假对照组精液内有精子,检测手术组精液内无精子,建模成功;进入下一步实验。、3个月;、血清抑制素B;:切取大鼠双侧睾丸,剔除附睾,分左右侧称重记录;,匀浆机制浆后离心,取上清液1ml检测睾丸组织内EGF含量。第二步:重复建立双侧射精管梗阻大鼠模型,行五子衍宗丸药物干预实验:,随机分为生理盐水组(对照组),五子衍宗丸组(实验组)其中实验组分低、高剂量组;。,检测血清睾酮及抑制素B;:切取大鼠双侧睾丸,剔除附睾,分左右侧称重记录;,匀浆机制浆后离心,取上清液1ml检测睾丸组织内EGF含量。结果:。,睾丸生精功能随时间延长而出现功能减退现象,1个月时睾丸重量较对照组增加,然血清抑制素B轻度下降,睾丸组织匀浆检测EGF含量呈降低。3个月时睾丸重量较对照组明显减轻,血清抑制素B含量降低,睾丸组织匀浆检测EGF含量降低明显,但血清睾酮含量在1个月及3个月的检测中却无明显差异。,双侧睾丸生精功能受到影响的条件下,3个月后EDO大鼠双侧睾丸重量、血清抑制素含量、血清睾酮含量、睾丸匀浆内EGF含量均较对照组(即生理盐水组)增加,且高剂量组效果明显。结论:。。,生精功能出现减退。。,能增加EDO大鼠模型血清睾酮含量。关键词:射精管梗阻;生精功能;五子衍宗丸Ejaculatory duct obstruction of rat testicular sperm production function and the influence of Wuzi Yanzong prescriptionof early interventionABSTRACTobjectives:To evaluate the effects of EDO and early intervention of WUZIYANZONG prescription ontestis functions of rats,and clarify the mechanisms of EDO infertile addition to providing experimental basis of WUZIYANZONG prescription,changing the current therapy for EDO as to significantly improve the natural fertility of these patients and save the cost of artificial procreation technology,and furthermore avoid ethical :The first step: male SD rats were randomly divided into two groups:bilateral EDO infertility group and sham-operated 2 weeks all rats were stimulated to ejaculate by electrical ejaculation semen of sham-operated g