文档介绍：pH-Responsive Poly(Ethylene Glycol) block-Polylactide Micelles for Tumor-Targeted Drug Delivery
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ABd, nm).HT: heat treatment. (D)Change in hydrodynamic diameter (d, nm) of PEG-a-PLA micelles in PBS solutions at different pH by DLS.
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Release profiles for PTX
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Release profiles for PTX from M-PTX, Mb-PTX and free PTX in PBS solutions at different pH in the presence of 1M sodium salicylate at 37 °C.
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Cytotoxicity of blank PEG-a-PLA micelles determined by CCK-8 analysis with different cell lines
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(A)Hela cells,(B)L-02 cells,(C) HUVEC.(D)Cellular internalization of PEG-a-PLA micelles fluorescently labeled with C6. M-C6 represents C6-labeled PEG-a-PLA micelles without treatment; Mb-C6 represents C6-labeled nonresponsive PEG-b-PLA micelles;“M-C6 pH x h〞denote the C6-labeled PEG-a-PLA micelles subjected to treatment with PBS at p H for x h. Scale bar: 50 μm.
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In vitro antitumor activity of M-PTX for Hela cells.
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(A) Cytotoxicity of the different PTX formulations determined by CCK-8 differences (P<) compared with Mb-PTX and free PTX are identified with *. (B)Cell cycle distributions of cells treated with the different PTX formulations for 48 h.
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Tumor-targeting efficacy of M-C6
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(A)In vivo time-dependent fluorescence images 0, 6, 24, and 48 h after the administration of M-C6 and free color bar(from red to blue)indicates changes in fluorescence radiation efficiency from high to low.(B)Quantification of the tumor-targeting characteristics of M-C6 and free C6.(C)Fluorescence images for tumor sections at 24 and 48 h postinjection of M-C6 and free C6. Scale bar: 100 μm.
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In vivo antitumor efficacy of M-PTX
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(A)Changes in the tumor volume and body weight of the Hela tumor-bearing nude mice receiving intravenous injections of M-PTX and free PTX.(B)Representative images for HE-stained tumor sections after treatment with M-PTX and free bar:100 μm.(C)PCNA and TUNEL