文档介绍：北京市海淀区产品质量监督检验所国家食品质量安全监督检验中心2011年06月23日质谱分析方法——定性与定量 China National Food Quality Safety Supervision and Inspection Center China National Food Quality Safety Supervision and Inspection CenterThe birth of Venus《LC and MS: A Match Made in Heaven》James Jorgenson,University of North Carolina59th,ASMS,June 5,Denver,USA China National Food Quality Safety Supervision and Inspection Center China National Food Quality Safety Supervision and Inspection CenterThe Arnolfini China National Food Quality Safety Supervision and Inspection Center China National Food Quality Safety Supervision and Inspection Center主要内容一、质谱基础及相关术语二、定性(确证)方法三、定量方法 China National Food Quality Safety Supervision and Inspection Center China National Food Quality Safety Supervision and Inspection EEI、CIESIAPCI、APPIMALDIDART-四级杆离子阱飞行时间磁质谱FT-ICR一、质谱基础及相关术语 China National Food Quality Safety Supervision and Inspection Center China National Food Quality Safety Supervision and Inspection Center各种离子化方法的使用范围选择的依据挥发性热稳定性极性NeutralNeutral101011101022101033101044101055Molecular WeightMolecular WeightIonicIonicAnalyte PolarityESI or MALDIAPCI/APPIGC/MS China National Food Quality Safety Supervision and Inspection Center China National Food Quality Safety Supervision and Inspection Center?质量范围?质谱仪所能测定的离子质荷比的范围?不同仪器?不同应用质谱仪主要性能指标?扫描速度?分辨率?色谱峰的数据点数目和峰宽、扫描时间有关 China National Food Quality Safety Supervision and Inspection Center China National Food Quality Safety Supervision and Inspection China National Food Quality Safety Supervision and Inspection Center China National Food Quality Safety Supervision and Inspection Center质谱仪主要性能指标磁质谱的分辨率定义最为严格:对两个相等强度的相邻峰,当两峰间的峰谷不大于其峰高10%时,则认为两峰已经分开,其分辨率:mmmmmR????1121磁质谱中,R不随m/z变化,在测定小分子时有优势eg. R=5000, 5000与5001分辨率 Ch