文档介绍：食品与发酵工业
Food and Fermentation Industries
ISSN 0253-990X,CN 11-1802/TS
BAE-BAG 菌株对底物、产物的耐受性与 H-型***水合酶菌株相仿；在以烟***
或者丙烯***为底物进行全细胞催化时，该菌株的催化速率与 L-型***水合酶菌株相仿，最终烟酰***产量可达到 516
g/L，是目前报道的最高产量；丙烯酰***产量比原始 BAE 或 BAG 菌株提高 %以上。该文成功协同两类***水合
酶，同时提高了合成烟酰***和丙烯酰***的催化反应速率及产量，对工业应用具有一定的指导意义。
关键词 ***水合酶；协同表达；烟酰***；丙烯酰***；全细胞催化
DOI: .11-1802/
Efficient production of nicotinamide and acrylamide with co-expression of two
kinds of nitrile hydratase
ZHAO Kaiyang, HUANG Weiyu, CAI Songjia, CHEN Yuanqing, WU Chaocheng, ZHOU Li , CHENG Zhongyi, ZHOU Zhemin
（School of Biotechnology, Jiangnan University, Wuxi 214122, China）
Abstract Nitrile hydratase (NHase) can hydrate nitrile substrates to generate more valuable amides, which are used
industrially in the production of important chemicals such as nicotinamide and acrylamide. These amides are very im portant
organic compounds, widely used in medicine, pesticide and chemical related fields. Most nitrile hydrases with high activity
have lower thermal stability, and the reaction temperature should be strictly controlled at lower level in industrial
application. In addition, nitrile hydrase has low