文档介绍：Reference: PA/PH/Exp. 10D/T (12) 6 ANP1
NOTE ON THE MONOGRAPH2
Ninhydrin-positive substances: TLC replaced by chroma10 mg add g of potassium nitrate R and 2 mL of sulfuric acid R. Heat on a water-bath for 20 min. Allow to cool. Add 5 mL of a 50 g/L solution of hydroxylamine hydrochloride R and allow to stand in iced water for 10 min. Add 9 mL of strong sodium hydroxide solution R. A violet-red or violet-brown colour
TESTS37
Appearance of solution. The solution is clear () and not more intensely coloured than reference solution BY6 (, Method II). 38
Dissolve g in a 103 g/L solution of hydrochloric acid R and dilute to 10 mL with the same
Specific optical rotation(): − to − (dried substance).40
Dissolve g in water R and dilute to mL with the same solvent. 41
Ninhydrin-positive substances. Examine by thin-layer chromatography (), using a TLC silica gel plate
Test solution (a). Dissolve g of the substance to be examined in a mixture of equal volumes of glacial acetic acid R and water R and dilute to 10 mL with the same mixture of
Test solution (b). Dilute 1 mL of test solution (a) to 50 mL with a mixture of equal volumes of glacial acetic acid R and water
Reference solution (a). Dissolve 10 mg of phenylalanine CRS in a mixture of equal volumes of glacial acetic acid R an