文档介绍：Using a Counting Chamber
For microbiology, cell culture, and many applications that require use of suspensions of cellsough so that the cells or other particles do not overlap each other on the grid, and should be uniformly distributed. To perform the count, determine the magnification needed to recognize the desired cell type. Now
systematically count the cells in selected squares so that the total count is 100 cells or so (number of cells needed for a statistically significant count). For large cells this may mean counting the four large corner squares and the middle one. For a dense suspension of small cells you may wish to count the cells in the four 1/25 sq. mm corners plus the middle square in the central square. Always decide on a specific counting patter to avoid bias. For cells that overlap a ruling, count a cell as "in" if it overlaps the top or right ruling, and "out" if it overlaps the bottom or left ruling.
Here is a way to determine a particle count using a Neubauer hemocytometer. Suppose that you conduct a count as described above, and count 187 particles in the five small squares described. Each square has an area of 1/25 mm-squared (that is, mm-squared) and depth of mm. The total volume in each square is ()x() = mm-cubed. You have five squares with combined volume of 5x() = mm-cubed. Thus you counted 187 particles in a volume