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降尿酸方对高尿酸血症肾病大鼠内皮功能的影响 郭亚芳.pdf

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降尿酸方对高尿酸血症肾病大鼠内皮功能的影响 郭亚芳.pdf

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降尿酸方对高尿酸血症肾病大鼠内皮功能的影响 郭亚芳.pdf

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文档介绍:网络首发时间:2022-04-22 18:52:34
网络首发地址:. 2022年 4月第 17卷na;2ShanghaiPudongHospital
AffiliatedtoFudanUniversity,Shanghai201399,China)
Abstract Objective:TostudytheeffectsofJiangniaosuanprescriptiononendothelialnitricoxidesynthase(eNOS)andendothe
lin(ET)inratswithhypoxanthineandadenineinduceduricacid(UA)nephropathy.Methods:Forty8weekoldmaleSDratsof
SPFgradewererandomlydividedintoanormalgroup,amodelgroup,anallopurinolgroup,andaJiangniaosuanprescriptiongroup,
with10ratsineachgroup.Thenormalgroupwasgiven2ml/dacidifiedwaterbygavagetwiceaday.Allgroupsweregivenhypo
xanthine1500mg/(kg·d)andadenine100mg/(kg·d)inthemorningfor4weeksexceptthenormalgroup.Themodelgroup
wasgiven2mL/dacidifiedwaterbygavageintheafternoonfor4weeks.Theallopurinolgroupwasgivenallopurinol(25mg/
100g)bygavageintheafternoon.TheJiangniaosuanprescriptiongroupwasgivenJiangniaosuanprescription16g/(100g·mL)
bygavageintheafternoon.Theratsweresacrificedafter4weektreatment,andtheserum creatinine(Scr),bloodureanitrogen
(BUN),andUAweredetected.Therenalfunctionofratswasobserved,andtherenalendotheliallesionwasmeasuredbyelectron
microscopy.Realtimequantitativepolymerasechainreaction(realtimePCR)wasusedtodeterminetheexpressionlevelsofmes
sengerribonucleicacid(mRNA)ofeNOSandET.TheexpressionsofeNOSandETw