文档介绍：Bioresource Technology 99 (2008) 8532–8536
Contents lists availabl cultured in a fed-batch mode with sucrose concentration maintained at ± g/L during 0–8 h and
Modeling
then batch culture was performed during 8–20 h with an initial sucrose concentration of 15 g/L. With
Two-stage culture
Hyaluronic acid the proposed two-stage culture strategy, HA production was increased to g/L compared with g/
Streptococcus zooepidemicus L in batch culture with the same total sucrose. The enhanced HA production by the proposed two-stage
culture strategy resulted from the decreased inhibition of cell growth and the increased transformation
rate of sucrose to HA.
Ó 2008 Published by Elsevier Ltd.
1. Introduction due to the strong inhibitions caused by the substrates of high con-
centration (Ding and Tan, 2006).
Hyaluronic acid (HA) is a linear glycosaminoglycan