文档介绍：: .
分子植物育种 RSCU 值>1，主要以 AT 结尾，且偏性较弱；中性绘图分析显示 GC12 与 GC3 的相关性低
(r=, P>)；ENC-plot 分析表明碱基的使用频率方面存在差异(U>A, G>C)；并且 16 个碱基组合(CGU,
GGU, UUA, UUG, ACU, GCA, CAU, AAA, AGU, UCU, GUA, GCU, AAU, GAU, CCA 和 CCU)被确定为最
优密码子，综上结果说明其 CUB 主要受选择效应的影响。因此，本研究阐明了芦笋 cp 基因组中 CUB 的
使用规律，为其叶绿体高效遗传转化的研究提供了参考依据。
关键词 芦笋; 叶绿体基因组; 密码子偏好性; 选择效应
Codon Usage Bias in the Chloroplast Genome of Asparagus Officinalis
L.
Sheng Wentao*
Department of Biological Technology, Nanchang Normal University, Nanchang, 330032
*Corresponding author, ******@
Abstract Based on the composition and characteristics of chloroplast (cp) genomic codon in Asparagus
officinalis L., we analyzed its codon usage bias (CUB) preferred use mode in order to provide a basis for further
genetic research. In this study, the effective number of codons (ENC), relative synonymous codon usage (RSCU),
ENC plot value and optimal codon of 52 coding gene sequences in cp genome were analyzed by Bioinformatics
and statistical methods. The results showed that the average GC content of codon bases 1, 2 and 3 in cp genome
showed a decreasing trend (GC1 (%)>GC2 (%)>GC3 (%); the size of ENC value ranged from
to , of which 42 ENC values were larger than 45; the RSCU value of 28 codons was larger than 1,
mainly ending with AT, and this bias was weak; Neutral mapping analysis showed that the correlation between
GC12 and GC3 was low (r=, P>); ENC plot analysis showed that there were differences in the
frequency of base use (U>A, G>C); And 16 base combinations (CGU, GGU, UUA, UUG