文档介绍：: .
河南工业大学在差异；使用 DEGseq 筛选差
异表达基因，并与七大功能数据库系统开展比对注释，共得到上调基因 8214 条，下调基因
3882 条，其中有 8638 个被注释到 GO 数据库，4310 个被注释到 KEGG 数据库；使用
DIAMOND 软件对差异表达基因进行蛋白互作分析，筛选出互作关系评分≥300 的网络关系
作图，得到 5 个 与白腐菌降级麦麸纤维相关的重要关键基因 、
、、、，为
深入研究白腐菌对麦麸膳食纤维的降解机制提供参考。
关键词： 白腐菌；降解；麦麸纤维；转录组；关键基因
Key genes of wheat bran degradation by white rot fungi
SONG Xin1, SU Hao2, JIANG Shiyu1, LI Li1*
1. College of Food Science and Engineering, Henan University of Technology, Henan Province
Zhengzhou 450001
2. Siziwang Banner Market Supervision Administration, Inner Mongolia Wulanchabu, 011800
Abstract: White rot fungi is one of the known microorganisms that can efficiently degrade
lignocellulose. In order to predict and screen the genes related to the degradation of wheat bran
fiber by white rot fungi, A. polytricha were cultured in liquid culture environment with
wheat bran fiber and glucose as single carbon source, the transcriptome of its were sequenced by
high-throughput sequencing technology, and the transcripts were compared and analyzed by go
and other databases. A total of clean data were obtained from the sequencing results of
BGI-500 high-throughput sequencing platform. After recombination and De-redundancy, a total of
31531 unigenes were obtained. The total length, average total length, N50 an