文档介绍：AMPK改善高脂饮食所致的早期 肾损害
•肥胖已成为全球性疾病
•到2030年美国肥胖人群（BMI大于30kg/m2）预计 将达到50%
•肥胖是代谢综合征的重要危险因素
•代谢综合征包括以胰岛素抵抗为基础的高血糖、 高血压、高甘油三 mice on STD. PT, proximal tubule.
Figure 1. HFD increases glomerular area and matrix accumulation. (A, B) Mice fed a HFD displayed large glomeruli and vacuolated cytoplasm (#). (C) Oil-red O staining on kidney sections on a HFD at 12 weeks: arrow shows the accumulated neutral lipid. (D) Quantitative analysis of glomeruli demonstrates increased mesangial matrix expansion, capillary filtration area, nuclei, and overall surface area. Immunofluorescence microscopy of cortical sections demonstrates increased collagen type I (E, F), collagen type IV (G, H), and fibronectin (I, J) in mice fed a HFD (F, H, and J) versus a STD (E, G, and I) for 12 weeks. Values are means ± SEM_ n = 5 in each group. Statistical analyses were performed by unpaired t test *P < versus mice on STD. PT, proximal tubule.
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Figure 2. HFD increases urine albumin, H2O2 and MCP-1. (A) Urine albumin/creatinine ratio (UACR) increases with HFD at 4,8； and 12 weeks. (B) Urine H2O2/creatinine level in mice on a HFD increases by 1 week and remains elevated during the 12-week period. (C) Urine MCP-1/creatinine level is increased in mice fed a HFD for 1 week. Values are means ± SEM. n = 5 in each group. Statistical analyses were performed by one-way ANOVA followed by Newman-Keuls test (A, B) *P < versus mice on a STD at
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Table 2. Effects of HFD on renal gene expressions at week 12 of experimental protocol
STD
HFD
Proinflammatory markers
TN Fa
±
±
IL-6
±
±
MCP-1
±
±
MIP-1a
±
±
IL-10