文档介绍：1 大黄、虫对药对雌孕激素负荷大鼠子宫肌瘤模型孕激素及其受体的影响作者:朱丽红,胡婷婷,严维娜【摘要】目的研究大黄、虫对大鼠子宫肌瘤模型孕激素及其受体的影响。方法将 70只 SD 大鼠随机分为 7组: 正常对照组、模型组、大黄虫小剂量组、大黄虫中剂量组、大黄虫大剂量组、大黄虫丸组、桂枝茯苓胶囊组, 采用雌孕激素负荷法建立大鼠子宫肌瘤模型, 放射免疫法测定大鼠血清孕激素(P) 水平, 免疫组织化学染色观察大鼠子宫平滑肌细胞孕激素受体(PR) 的表达。结果模型大鼠血清中 P 水平,子宫平滑肌细胞PR 的表达明显高于正常大鼠, 大黄虫可降低血清中 P 水平, 抑制平滑肌细胞 PR 的表达。结论 P及 PR 在子宫肌瘤生长中起重要作用, 这可能是大黄虫治疗子宫肌瘤的机理之一。【关键词】大黄、虫; 子宫肌瘤模型; 孕激素; 孕激素受体 2 Abstract : ObjectiveTo study the effects of Da Huang and Zhe Chong on the concentration of progesterone and its receptor in the experimental rats with uterine lEiomyomas. Methods70 female Sprague-Dawley rats were randomly divided into 7 groups: control group, model group, low dosage group of Da Huang and Zhe Chong, medium dosage group of Da Huang and Zhe Chong , higher dosage group of Da Huang and Zhe Chong , Da Huang Zhe Chong Wan group, Gui Zhi Fu Ling Capsula group. The animal model of uterine lEIomyomas was induced by estrogen and progesterone load method. The serum concentration of progesterone was measured by radioimmunoassay (RIA), and the expressions of progesterone receptor in smooth muscle cells was detected by immunohistochemistry. pared with control group, the concentration of progesterone and expression of progesterone receptor in smooth muscle cells in model group were changed significantly(P ). Da Huang and Zhe Chong were demonstrated to reduce the concentration of progesterone, suppress the expression of progesterone concentration of progesterone and progesterone receptor play important role in the growth of uterine leiomyomas, and it may be one of reasons by which Da Huang and Zhe Chong treat 3 uterine leiomyomas. Key words : Da Huang and Zhe Chong; Models of the uterine leiomyomas; Progesterone; Progesterone receptor 子宫肌瘤是女性***官中最常见的良性肿瘤,也是人体最常见的肿瘤之一,它好发于生育年龄(30 ~ 50岁) 的妇女,以 40~ 50 岁最多见, 20 岁以下少见[1] 。虽恶变率极低, 但所引发的子宫出血、腹痛、不孕、贫血等严重影响妇女身心健康。子宫肌瘤确切的发病原因至今尚不清楚, 但与卵巢性激素密切相关, 是公认的激素依赖性肿瘤。中医学中无“子宫肌瘤”的病名, 根据其临床表现,属“癥瘕”范畴, 即妇女下腹包块,伴有或痛或胀或满或***出血溢液等症状的病证, 其病机关键是瘀血内阻。大黄、虫