文档介绍：1 酸枣仁功效成分测定及改善睡眠保健功能的研究作者: 黄维, 金邦荃, 程光宇, 杨云峰, 冯波, 吴京燕【摘要】目的研究酸枣仁功效成分的含量对小鼠睡眠的改善作用及改善睡眠的初步机理。方法用化学方法测定酸枣仁粗多糖、总黄***和总皂苷的含量, 高效液相色谱法测定酸枣仁皂苷 A 的含量。通过直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下催眠剂量实验和巴比妥钠睡眠潜伏期实验, 比较低、中、高3 个剂量( 570 ,1 000 及2 000 mg/kg ) 酸枣仁粉剂对小鼠睡眠的影响, 并且用荧光法测定小鼠脑中 5- 羟色***含量。结果酸枣仁粉剂总皂苷的含量为 g/100 g ,酸枣仁皂苷 A 的含量为 g/100 g ,总黄***的含量为 g/100 g。中剂量样品(1 000 mg/kg ) 具有改善睡眠的作用, 酸枣仁改善睡眠与小鼠脑中 5- 羟色***含量具有相关性。结论酸枣仁粉剂功效成分的含量较高,具有改善睡眠的作用,改善睡眠与小鼠脑中 5- 羟色***含量有关。【关键词】酸枣仁; 功效成分; 改善睡眠; 5- 羟色*** 2 Abstract : ObjectiveTo study the content of position of Semen Ziziphi Spinosae and its effect of improving content of coarse amylose, total flavone and total jujuboside were measured by chemical processes. The content of jujubosideA was measured by HPLC. The effect of sleep improvement of the three dosages(570 mg/kg,1000 mg/kg and 2000 mg/kg)of Semen Ziziphi Spinosae was studied by the experiments of sleep-induced directly, prolonging sleeping time induced by pentobarbital sodium, affecting mouse sleeping rate under the threshold hypnogenesis dosage of pentobarbital sodium and affecting sleeping delitescence induced by tobarbital sodium. The content of 5-HT in the brain of mouse was measured by fluorescence assay. ResultsThe content of total jujuboside in Semen Ziziphi Spinosae was g/100 g, jujubosideA was g/100 g, and total flavone was g/100 g. The middle dosage (1 000 mg/kg) could improve the sleep quality, and sleep improvement had high relativity with 5-HT. ConclusionSleep can be improved by Semen Ziziphi Spinosae, and it has relation with the high content of the position and the content of 5-HT in the brain of mouse. 3 Key words : Semen Ziziphi Spinosae ; position ; Sleep improvement ; 5-HT 酸枣仁 Semen ziziphi spinosae 为中医传统安神药材,《中国药典》 2000 版第Ⅰ部记载为鼠李科植物酸枣 Ziziphus jujuba . spinosa(Bunge) Hu ex . Chou 的干燥成熟种子, 其味酸性平, 归肝、胆、心经主要用于治疗虚烦不眠, 惊悸多梦, 体虚多汗, 津伤口渴等症。酸枣仁的药理作用主要有镇静、催眠、镇痛、抗惊厥、降温、降压、降脂、抗缺氧、增强免疫功能以及对心脏的保护作用。随着社会的发展, 失眠已经成为困扰人们生活的重要因素。改善睡眠的研究很多集中在西药如