文档介绍：淋巴细胞提取
一实验目的：小鼠脾淋巴细胞提取
二实验对象：B/C小鼠
三实验器材：
：淋巴细胞分离液、 1640培养基
：无菌培养皿、 200目尼龙网（裁成 90mm*90mm正方形，灭菌）、10mL玻璃注射
器P
可明显抑制肿瘤细胞的生长， 增加肿瘤浸润 CD4+、CD8+细胞的数量。LBP高剂量组CD4+
和CD8+细胞数量的百分比高于模型组 (P<)。LBP低剂量和高剂量组肿瘤浸润 DCs数量
及B7损1表达均较模型组升高，但差异无统计学意义。结论： LBP的抗肿瘤作用可能与恢 复荷瘤小鼠TIL中CD4+、CD8+的细胞数量、解除机体的免疫抑制状态及增强机体的抗肿 瘤免疫功能有关。LBP能否恢复荷瘤机体 DCs的表型及功能尚待进一步研究。
〔关键词〕枸杞多糖；免疫抑制；淋巴细胞亚群；肿瘤浸润淋巴细胞；树突状细胞
Effects of Lycium barbarum polysaccharide on tumor microenvironment T 拟 lymphocyte subsets and dendritic cells in H22 拟 bearing mice
HE Yan 拟 Li, YING Yi, XU Yan 拟 Li, SU Jun 抵( Fang, LUO Hui, WANG Hui 叔 Feng
(Department of Pathology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510405, China; Department of Hematology, Guangzhou Municipal First People ' s Hospital, Guangzhou, Guangdong Province 510180, China)
ABSTRACT Objective: To study the effects of Lycium barbarum polysaccharide (LBP) on tumor microenvironment T 拟 lymphocyte subsets and dendritic cells in H22 拟 bearing mice and the mechanisms for intervention of tumor immune escape by LBP. Methods: H22 拟 bearing mice were given LBP orally for two weeks. T 叔 lymphocyte subsets and the phenotypes of dendritic cells in tumor 拟 infiltrating lymphocytes (TIL) were detected by flow cytometry (FCM). Results: LBP could significantly increase the numbers of CD4+ and CD8+ T cells in TIL as compared with those in