PCR Protocols-[David Stirling (Editor) John M S Bartlett (Editor), John M S Bartlett, David Stirling].pdf

文档介绍：Methods in Molecular Biology TMTM
Volume 226
PCRPCR
ProtocolsProtocols
SSECONDECOND EEDITIONDITION
EditedEdited byby
JohnJohn . . BartlettBartlett
DavidDavid StirlingStirling
Contents

1. A Short History of the Polymerase Chain Reaction 3
John M. S. Bartlett and David Stirling
2. PCR Patent Issues 7
Peter Carroll and David Casimir
3. Equipping and Establishing a PCR Laboratory 15
Susan McDonagh
4. Quality Control in PCR 20
David Stirling
5. Extraction of Nucleic Acid Templates 27
John M. S. Bartlett
6. Extraction of DNA from Whole Blood 29
John M. S. Bartlett and Anne White
7. DNA Extraction from Tissue 33
Helen Pearson and David Stirling
8. Extraction of DNA from Microdissected Archival Tissues 35
James J. Going
9. RNA Extraction from Blood 43
Helen Pearson
10. RNA Extraction from Frozen Tissue 45
John M. S. Bartlett
11. RNA Extraction from Tissue Sections 47
Helen Pearson
12. Dual DNA/RNA Extraction 49
David Stirling and John M. S. Bartlett
13. DNA Extraction from Fungi, Yeast, and Bacteria 53
David Stirling
14. Isolation of RNA Viruses from Biological Materials 55
Susan McDonagh
15. Extraction of Ancient DNA 57
Wera M. Schmerer
16. DNA Extraction from Plasma and Serum 63
David Stirling
17. Technical Notes for the Detection of Nucleic Acids 65
John M. S. Bartlett
18. Technical Notes for the Recovery and Purificationof PCR Products from Acrylamide Gels 77
David Stirling
19. PCR Primer Design 81
David L. Hyndman and Masato Mitsuhashi
20. Optimization of Polymerase Chain Reactions 89
Haiying Grunenwald
21. Subcycling PCR for Long-Distance Amplificationsof Regions with High and Low
Guanine–Cystine Content---- Amplification of the Intron 22 Inversion of the FVIII Gene
David Stirling 101
22. Rapid Amplification of cDNA Ends
i
Xin Wang and W. Scott Young III 105
23. Randomly Amplified Polymorphic DNA Fingerprinting--The Ba