文档介绍：肉苁蓉对败血症休克大鼠胸腺细胞凋亡的影响
【摘要】目的探讨肉苁蓉提取物对败血症休克大鼠胸腺细胞凋亡的影响及其机制。方法 60只大鼠随机分为实验组、空白对照组和对照组,各20只,空白对照组分离盲肠后不做结扎、穿孔处理,实验组及对照组行盲肠结扎穿孔术(CLP)建立大鼠败血症休克模型。 g/kg,%氯化钠注射液。于术后12、24 h取材。流式细胞仪检测各组胸腺细胞凋亡率和线粒体膜电位,分光光度法测定细胞SOD活性、MDA含量。结果实验组CLP术后12、24 h胸腺细胞凋亡率均低于对照组(P&lt;),线粒体膜电位显著上升(P&lt;),细胞超氧化物转化酶(SOD)活性上升、丙二醛(MDA)含量下降与对照组比较差异均有统计学意义(P<)。结论肉苁蓉提取物对败血症休克时胸腺细胞有保护作用,其机制可能是提高SOD活性,降低MDA含量,清除自由基反应,维持了细胞线粒体膜电位。
【关键词】肉苁蓉出血性败血症细胞调亡线粒体超氧化物歧化酶丙二醛动物实验
Effect of Roucongrong on thymus cell apoptosis in rats ia
【Abstract】 Objective To observe the effect and mechanism of extract of Roucongrong on thymus cell apoptosis in rats ia. Methods 60 rats ly divided into experimental group, blank control group and control group, 20 rats in each group. The rats in blank control group separation, ental group and control group ed by cecum ligation perforation (CLP) to set up rat model ia. The rats in three groups ens itochondrial membrane eter and the activity of SOD and content of MDA ined by spectrophotometry. Results The thymus cell apoptotic rate at 12 h and 24 h after the CLP operation in experimental group itochondrial membrane us cell in rats ia, its mechanism might be related itochondrial membrane.
【Key ia; Thymus cells; Chondriosome
败血症休克是临床上常见的危重病症,病死率居高不下。在其病理损伤中,胸腺细胞凋亡的作用日益受到重视。T淋巴细胞的过早凋亡,是引起机体免疫抑制的主要原因之一。本实验探讨了肉苁蓉对盲肠结扎穿孔术(CLP)诱导的败血症休克大鼠模型中胸腺细胞凋亡率及细胞超氧化