文档介绍：: .
食品工业科技，考察 AMF 的抗氧化活性；以胆酸钠、牛黄胆酸钠、甘氨胆酸钠为结合对象，考察 AMF 的胆酸盐
结合能力。结果表明，AMF 的最优提取工艺为：液料比 61 mL/g、乙醇浓度 60%、超声功率 100 W、超声
时间 59 min，黄酮得率为 %±%，与模型预测值（%）接近。最优工艺制备的 AMF 对 DPPH•、
+
ABTS •清除率较强，其 IC50 值分别为 、 mg/mL；质量浓度为 mg/mL 时，总还原能力较好，其
吸光值为 ；AMF 对胆酸钠、牛黄胆酸钠、甘氨胆酸钠具有较强结合能力，结合率 IC50 值分别为 、
、 mg/mL。综上，AMF 有较强的抗氧化和胆酸盐结合能力。本研究可为 AMF 进一步开发天然抗
氧化剂和降血脂药物提供依据。
关键词：黑果腺肋花楸叶；黄酮；超声波辅助提取；工艺优化；抗氧化；结合胆酸盐能力
Optimization of Extraction Process of Flavonoids from Aronia
melanocarpas’ Leaves and Analysis of Their Antioxidant and
Bile Salt Binding Capacity
HE Xuhua, SHI Zhijiao, WANG Anna, ZHAO Chunfang, LIU Yun, KAN Huan
(College of Life Sciences, Southwest Forestry University, Kunming 650224, China)
Abstract: In this study, the antioxidant and bile salt binding activities of flavonoids from Aronia melanocarpas’
leaves (AMF) by ultrasound-assisted extraction. Based on single factors (liquid-solid ratio, ethanol concentration,
ultrasonic power and ultrasonic time) and response surface method, the extraction process of AMF was optimized
with flavonoid yield as the index. The antioxidant activity of AMF was evaluated by DPPH•, ABTS+• scavenging
rate and reduction power; the bile salt binding ability of AMF was investigated by using sodium cholate, s