中药栀子总环烯醚萜苷的含量测定.pdf

文档介绍：安徽农业科学,Journal of Anhui Agri. Sci. 2011,39 21 12859 - 12860 责任编辑刘群燕责任校对况玲玲
中药栀子总环烯醚萜苷的含量测定
王赟,李朝晖,华春* ,
南京晓庄学院生物化工与环境工程学院江苏南京 211171
摘要[目的]建立栀子药材中栀子总环烯醚萜苷含量的测定方法。[方法]采用紫外分光光度法,以甲醇为空白,在波长 238 nm 处测定
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栀子总环烯醚萜苷含量。结果栀子苷在 7. 82 ~ 31. 30 μg /ml 范围内线性关系良好 r = 0. 999 7 平均回收率为 109. 20% RSD 值为
4. 73% n = 5 。江西产药材中栀子总环烯醚萜苷含量最高。[结论]该法简便、准确、重复性好,可作为栀子的质量控制方法。
关键词栀子总环烯醚萜苷紫外分光光度法
中图分类号 S567 文献标识码 A 文章编号 0517 - 6611 2011 21 - 12859 - 02
Determination of Total Iridoid Glycoside in FRUCTUS GARDENIAE
WANG Yun et al College of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing,Jiangsu 211171
Abstract [Objective]To establish the determining method for total iridoid glycoside in FRUCTUS GARDENIAE. [Method]Content of total
iridoid glycoside was determined by ultraviolet spectrophotometry at the wavelength of 238 nm with methanol as blank. [Result]In the range
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of 7. 82 - 31. 30 μg /ml geniposide showed a good linear relationship r = 0. 999 7 with mean recovery rate of 109. 20% and RSD value of
4. 73% n = 5 . FRUCTUS GARDENIAE sampled from Jiangxi Province was highest in total iridoid glycoside. [Conclusion]Simple in oper-
ation and good in repeatability,the method established in this work could be used to the quality control of FRUCTUS GARDENIAE.
Key words FRUCTUS GARDENIAE Total iridoid glycoside UV spectrophotometry
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