文档介绍：葎草抗结核分枝杆菌研究
作者:陈伟光林霞睢凤英尹秀屠婕红盛静黄佳
【摘要】目的研究葎草提取物抗结核菌活性及有效部位。方法以体外抑菌实验筛选葎草抗结核活性部位,采用试管倍比稀释法测定葎草提取物最低抑菌浓度(MIC),评价其体外抗菌活性。结果葎草的正丁醇萃取部位对结核分枝杆菌标准株H37 RV 及临床分离的敏感菌株具有较明显的抗菌作用,粗提物的MIC 为125 μg· ml-1 ,高浓度1 mg· ml-1 时有杀灭作用。结论葎草提取物对结核杆菌具有明显的抗菌活性,有进一步研究开发的价值。
【关键词】葎草结核分枝杆菌抗菌活性
Abstract:ObjectiveTo observe the activity of extracts from Humulus scandens against tuberculous bacillus and to find out the active method of bacteriostasis in vitro was used to evaluate the inhibitory effects against tuberculous bacillus of the extracts from Humulus of the extracts were measured by the means of double dilution .ResultsThe N -butanol extract had obvious antibacterial activity to H 37 RV and the sensitive strain which separated from clinic .MIC of the extract was 125 μg· ml-1 and the high levels (1 mg· ml-1)of the extract had a killing extracts of have obvious antimicrobial activity in is ulus scandens (Lour.) Merr.; Tuberculous bacillus; Bacteriostasis
近年来,全球结核病疫情再度上升,受到了世界各国的关注。全球1/3 人口(约20 亿)感染过结核菌,全世界现有结核病人2 000万,每年还会新发生800 ~1 000 万肺结核病人,死亡约200万人。2000 年全国结核病流行病学调查的结果显示,我国有4~5 亿人受到过结核杆菌的感染,有各类肺结核病人约800 万,每年新增传染性肺结核患者150 万人,有13 万人死于结核病。目前临床上常用的抗结核药物大多对肝、肾脏有较大的毒性,且易产生耐药性。因此,近年来从天然资源中寻找安全有效的抗结核药物,已引起越来越广泛的重视。葎草是一种民间草药,为桑科植物Humulus scandens (Lour.) 。含青兰苷、天门冬素、挥发油、鞣质及树脂、木犀草素葡萄糖苷、大波斯菊苷、牡荆素等黄***类成分,具清热解毒、利尿消肿等功效,