文档介绍：Protection Induced by Plasmodium falciparum MSP142 Is
Strain-Specific, Antigen and Adjuvant Dependent, and
Correlates with Antibody Responses
Jeffrey A. Lyon1, Evelina Angov1*, Michael P. Fay2, JoAnn S. Sullivan3, Autumn S. Girourd1, Sally J.
Robinson1, Elke S. Bergmann-Leitner1, Elizabeth H. Duncan1, Christian A. Darko1, William E. Collins3,
Carole A. Long4, John W. Barnwell3
1 Division Malaria ine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America, 2 Biostatistics Research Branch,
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America, 3 Division of Parasitic Diseases, Malaria
Branch, National Center for Zoonotic, Vector-Borne and Enteric Disease, Centers for Disease Control, Atlanta, ia, United States of America, 4 Department of Malaria
and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
Abstract
ination with Plasmodium falciparum plete Freund’s adjuvant (FA) followed by MSP142/plete FA is the
only known regimen that protects Aotus nancymaae monkeys against infection by erythrocytic stage malaria parasites. The
role of adjuvant is not defined; plete FA cannot be used in humans. In rodent models, immunity is strain-
specific. We inated Aotus monkeys with the FVO or 3D7 alleles of MSP142 expressed in Escherichia coli or with the FVO
allele expressed in baculovirus (bv) combined plete and plete FA, Montanide ISA-720 (ISA-720) or AS02A.
Challenge with FVO strain P. falciparum showed that suppression of cumulative day 11 parasitemia was strain-specific and
could be induced by E. coli expressed MSP142 bination with FA or ISA-720 but not with AS02A. The coli42-FVO
antigen induced a stronger protective effect than the bv42-FVO antigen, and FA induced a stronger protective effect than
ISA-720. ELISA antibody (Ab) respon