文档介绍：第 17 卷第 7 期中国实验方剂学杂志 Vol. 17,No. 7
2011 年 4 月 Chinese Journal of Experimental Traditional Medical Formulae Apr. ,2011
HPLC 测定芩翘抗感颗粒中黄芩苷的含量
费超1 ,陈然2 ,曹杰1 ,张海鸣1 ,方睿1 ,刘子沐1 ,杜树山1*
1. 中药资源保护与利用北京市重点实验室,北京 100875
2. 北京超微生物制品有限责任公司,北京,100007
[摘要] 目的建立芩翘抗感颗粒中黄芩苷的高效液相含量测定方法方法采用,
。 Diamonsil C18 4. 6 mm × 250 mm 5
μm 色谱柱,以甲醇-0. 1% 磷酸溶液48∶ 52 为流动相,流速 1 mL·min - 1 ,检测波长 280 nm,柱温 30 ℃。结果黄芩苷含量测定
的线性范围 0. 49 ~ 2. 46 μg r = 0. 999 9 ,精密度试验 RSD 0. 25% ,平均加样回收率 100. 54% ,RSD 1. 89% 。结论方法快速、
简便、准确、重复性较好,结果可靠,可为芩翘抗感颗粒的质量评价提供有效手段。
[关键词] 芩翘抗感颗粒黄芩苷高效液相色谱法
[中图分类号] R284. 1 [文献标识码] A [文章编号] 1005-9903201107-0063-03
Determination of Baicalin in Qinqiao Kanggan Granules by HPLC
FEI Chao1 ,CHEN Ran2 ,CAO Jie1 ,ZHANG Hai-ming1 ,FANG Rui1 ,LIU Zi-mu1 ,DU Shu-shan1*
1. Protection and Utilization of Traditional Chinese Medicine of Beijing
Area Major Laboratory,Beijing 100875,China
2. Beijing Chaowei Pharmaceutical Co. Ltd. ,Beijing 100007,China
[Abstract] ObjectiveTo estalish an accurate method for the determination of baicalin in Qinqiao Kanggan
, ,
granules. Method Diamonsil C18 4. 6 mm × 250 mm 5 μm column in an oven at 30 ℃ was need with a mobile
phase consisting of mathanol-0. 1% phosphoric acid48∶ 52 and a UV detector at 280 nm,the flow rate was 1. 0
mL·min - 1 . Result The linear ranges of baicalin was 0. 49 - 2. 46μg r = 0. 999 9 . The RSD of measurement
precisio