文档介绍：山东农业大学
硕士学位论文
白首乌的组织培养及其生理生化分析
姓名:徐飞
申请学位级别:硕士
专业:遗传学
指导教师:于元杰
2011-06-08
山东农业大学硕士学位论文
中文摘要
分别以白首乌的叶片、茎段、种子和根为外植体,通过愈伤诱导途径和直接诱导丛
生芽途径建立了再生体系,获得的主要结果如下:
1. 愈伤组织诱导:不同培养基及激素组合对愈伤组织的形成影响很大,白首乌愈伤
组织诱导阶段宜选用 MS 作基本培养基,而生长素 2,4-D 是愈伤组织形成的关键因素。
以种子、茎段、叶片为外植体均诱导出愈伤组织,但是不同外植体在出愈率和愈伤生长
方面差异较大,其中茎段是最理想的外植体;筛选出最佳诱导培养基为:2,4-
-1+6- -1 + -1。
2. 愈伤组织分化成苗:将生长较好的愈伤组织转接到分化培养基上,愈伤组织逐渐
变绿,并形成很多突起,进而形成多芽体。筛选出最佳分化培养基为 mg·L-1+
mg·L-1,分化率高达 %。再生苗接种到生根培养基上, 8 号培养基生根效果
最好,生根率高达 %,且根的长势强。最佳生根培养基为 1/2MS+-1+ NAA
-1。其中 NAA 是影响根的形态分化的关键因子。
3. 诱导丛生芽建立再生体系:以根、茎尖和带腋芽茎段为外植体都可以诱导出芽苗,
诱导芽苗的最佳培养基为 MS+-1+NAA -1,诱导率最高为 %;
MS+ -1 + -1 是芽苗继代的最佳培养基,增殖系数最高,达到 。
4. 琼脂浓度对愈伤组织褐化和玻璃化的影响:琼脂浓度为8 g/L 时,愈伤组织的增
殖系数最高,,愈伤组织生长最好。愈伤褐化率随着琼脂浓度的增加而增大,琼
,培养基较硬,不适合愈伤的生长,%。琼脂浓度太低
()时愈伤组织容易玻璃化,%。
关键词:白首乌; 组织培养; 愈伤组织; 生理生化
1
白首乌的组织培养及其生理生化分析
Tissue Culture in Cynanchum auriculatum and its
Physiological and Biochmical Chacteristics
Abstract
Two regeneration systems were established respectively on Cynanchum auriculatum
through two approachs: one was inducement and differentiation of callus with leaf, stems ,
seeds and radicles as explants and the other was inducement of clustered shoot. The main
results can be summarized as follows:
1. Inducement of callus: The effects of different medium and binations on
callus formation were greatly,the best basic medium is MS ,and auxin 2,4-D is a key
factor in callus formation. Callus could be induced by different explants including leaf,
stem and seed, but induce ratio and growth form of callus were different from each other.
It was proved that stem was the best explant for inducing callus.
2. Inducement and proliferation of buds: The callus turned green and formatted many buds
after transferred into the culture medium, and then clustered s