文档介绍：The specific genes of an formation
1. Adventitious root formation of specific genes
HRGPnt3, PS-IAA4/5/6 increased expression at the adventitious root primordia formation stage.
PXA1 / phenotypic expression of this gene mutation significantly reduced the number of lateral roots.
OsCKI1 promotes the occurrence of adventitious roots
Using the rooting gene rol of agricultural pole of bacterial T-DNA to transform plant cells, could improve the efficiency of rooting of transgenic plants .
2. The specific genes of adventitious buds formation.
Shoot apical meristem (SAM): is located above of the leaf primordia, and has the vertical structure and radiation structure. The radiation structure includes two parts,the central area and the peripheralzone. the peripheralzone differentiated leaf, bud and the outer tissue of the stems.
SAM has a strong regeneration ability
3 .Gene regulates the SAM.
By the study of Arabidopsis mutants We found that several genes regulate the SAM of the induction and maintenance, including: STM, WUS, CLV1, CLV3.
STM belongs to the KNOX gene family, encoding the transcription factors of the homologous domain related tothe maize KNOTTED1 proteins.
STM of low activity is not conducive to maintain the SAM.
The SAM of STM maintaining may be regulated by the expression of suppressor gene (AS1). AS1 expressed in leaves primordia. STM inhibited the expression of AS1 gene, The antagonism of STM and AS1 can separat SAM from leaves primordia .
WUS gene encodes transcription factor of the homologous domain. Expression is restricted to the original cells of the central area.
The fouction of the CLV1 and CLV3 gene is limiting the size of the central area.
CLV1 encodes the protein of receptor kinase and plays a role in transmission of information.
CLV3 encodes a small extracellular protein molecules, is the ligand of CLV1.
The expression of CLV1 is confined to the elementary body of the central area, CLV3 expression is mainly in the tunica of the central area