文档介绍：鸭环磷酸腺苷ELISA说明鸭环磷酸腺苷原理,cAMELISA步骤说明
鸭环磷酸腺苷ELISA说明鸭环磷酸腺苷原理cAMELISA步骤说明标本处理要求: 收集标本前必须清楚要检测的成份是否足够稳定。对收集后当天进行检测的标本,储存在 4 ℃备用,如有特殊原因需要周期收集标本,将标本及时分装后放在-20 ℃或-70 ℃条件下保存。避免反复冻融。标本 2 -8 ℃可保存 48 小时, -20 ℃可保存 1 个月。-70 度可保存 6 个月。部分激素类标本需添加抑肽酶。,
鸭环磷酸腺苷ELISA说明鸭环磷酸腺苷原理steps
1. Before use, all reagents thoroughly incorporated. Don't make the liquid produces large amounts of foam, so as not to add sample with lots of bubbles and error on the sample.
2. According to the sample under test number plus the number of standard decided the number of panel. Each standard and blank hole advice after hole. To be decided according to the number of each sample, can use the hole after hole as far as possible to do.
3. Join the dilution after 50 ul standard joined sample under test in reaction hole, 50 ul in reaction to the hole.
Immediately add 50 ul biotin labeled antibody. Cover the diaphragm, oscillation and mixed gently, 37 ℃ incubate for 1 hour.
4. Dump hole liquid, each hole filled with detergents, oscillation, 30
seconds to jilt to detergents, pat dry with a blotting paper. Repeat this three times. If use washing machine washing, washing more often.
5. 80 per hole to join ul affinity chain element - HRP enzyme, oscillation and mixed gently, temperature of 37 ℃) for 30 minutes.
6. Dump hole liquid, each hole filled with detergents, oscillation, 30 seconds to jilt to detergent