文档介绍：不同品系黄连的核型分析
摘要:采用压片法对黄连(Coptis chinensis Franch.)不同品系植株的染色体数目进行统计及核型分析。结果显示,黄连3个品系的染色体数目相同,均为2n=18;但核型公式和核型类型有所差异,大花叶黄连和有光叶黄连的核型公式均为2n=18=18m,小花叶黄连核型公式为2n=18=6m+10sm+2tm;大花叶黄连和有光叶黄连核型类型均为1A,小花叶黄连核型类型为3B。黄连不同品系间染色体进化程度有所差异,可以为栽培上筛选良种和遗传育种提供参考。
关键词:黄连(Coptis chinensis Franch.);染色体;核型分析
中图分类号:+2 文献标识码:A 文章编号:0439-8114(2017)11-2079-03
DOI:-
Abstract: Chromosome number was calculated and karyotype was analyzed on different varieties of Coptis chinensis using the method of squash. The results show that three varieties of Coptis chinensis have the same number of chromosomes and all are 2n=18. Karyotype formula and karyotype type are differences
,karyotype formula of Dahuaye and Youguangye are 2n=18=18m,karyotype formula of Xiaohuaye is 2n=18=6m+10sm+2tm. karyotype type of Dahuaye and Youguangye are 1A,karyotype type of Xiaohuaye is 3B. There are several evolution degree differences for different strains of C. chinensis,which can provide some prerequisites for improving selection of variety.
Key words: Coptis chinensis Franch.; chromosome; karyotype analysis
?S连(Coptis chinensis Franch.)又名味连、川连、鸡爪黄连、光莲,为毛茛科黄连属多年生草本植物,具有泻火解毒、清热燥湿和良好的抗菌功效[1],因而备受国内外药学工作者的关注。黄连主产于四川、湖北、贵州、陕西等地区,以栽培为主,在长期的栽培过程中植株形态有参差不齐的现象,出现多种变异类型[2]。根据其叶面颜色、叶片大小、叶面光泽等,黄连分成不同品系(大花叶、小花叶、有光叶等)。
核型是遗传物质在细胞水平上的表征,与外部形态性状相比,核型受外界环境影响小,是推测物种起源、演化、分类和鉴别的重要依据[3,4]。近年来