文档介绍：目录
摘要 I-II
1前言 1
2 材料与仪器 2
2
2
实验动物 2
3 方法 3
样品制备 3
剂量选择与受试物给予方式 3
小鼠淋巴瘤TK基因突变实验(MLA试验) 4
鼠伤寒沙门氏菌/回复突变试验(Ames试验) 6
骨髓微核试验 7
4 结果 8
MLA实验结果 8
Ames实验结果 9
10
5 讨论 11
参考文献 13
综述 14
致谢 19
芦荟通畅胶囊的遗传毒性研究
摘要:目的评价芦荟通畅胶囊的遗传毒性;方法采取小鼠淋巴瘤TK基因突变试验(Mouse Lymphoma assay TK gene mutation test)、鼠伤寒沙门氏菌/回复突变试验(Salmonella Typhimuriun / Reverse mutation assay,Ames Assay)以及骨髓细胞微核试验评价芦荟通畅胶囊的遗传毒性。设芦荟通畅胶囊提取液剂量组20、10、5、、,在添加S9和不添加S9条件下,通过Ames试验和TK试验对其进行体外遗传毒性评价;、、,通过骨髓细胞微核试验对其进行遗传毒性评价。Ames试验中,设置计数TA97a、TA98、TA100、TA102四个标准测试。菌株在四个浓度下,于37℃、48 h培养后回复突变菌落数;MLA试验中,计算大小集落的孔数、平板效率PE %、基因突变频率MF和小集落百分率SC%;骨髓细胞微核试验中,每只动物计数1000个嗜多染红细胞,其微核发生率以含微核的PCE千分率计。结果芦荟通畅胶囊提取物Ames试验受试物各剂量组回变菌落数均未超过溶剂对照回变菌落数2倍,未出现剂量-反应关系;MLA试验中,芦荟通畅胶囊提取物各剂量组MF值与溶剂对照组相比均无显著性差异,未出现剂量-反应关系;骨髓细胞微核试验中受试物各剂量组的微核发生率差异无显著性(P>),而雌雄小鼠阳性对照组均明显高于阴性对照组(P<)。结论芦荟通畅胶囊提取物Ames试验、MLA试验和骨髓细胞微核试验中均未被检测出遗传毒性。
关键词:芦荟通畅胶囊;小鼠淋巴瘤TK基因突变实验;鼠伤寒沙门氏菌/回复突变试验;骨髓细胞微核试验
ic toxicity studies of ALOE RELAX CAPSULE
Abstract: Objective To evaluate the ic toxicity of aloe relax capsule; Methods adopt Mouse Lymphoma TK gene mutation test, Salmonella typhimurium/back mutation test and bone marrow cells micronucleus test evaluation of ic toxicity of aloe relax capsule. Set aloe relax capsule extract dose group of 20, 10, 5, , mg/dish, under the condition of adding the integrated and not add the integrated, by Ames test and TK test on the in vitro ic toxicity evaluation; Put aloe relax capsule suspension liquid dosage group , , g/ml, through bone marrow cell micronucleus test on the ic toxicity assessment. Ames test, set the count TA97a, TA98, TA100, TA102 four standard test. Strains under the four levels, at 37 ℃, 48 h after training number back mutation colonies; MLA experiment, calculating the number of holes size colony, plate efficiency PE % percentage, gene mutation frequency MF and small colony SC %; Micronucl