文档介绍：苦杏仁甙检测方法
西安源森生物科技有限公司 400-088-6586
1. Instrument 设备
a HPLC-9a (Shimadzu) b Sartorius electronic balance (Germany) 电子称
c. KQ-----model 100, Washing device of ultrasonic wave 100-KQ 超声波洗涤设备
2. Condition 条件 Chromatographic Column 色谱柱? ROM-150 ODS-c18(×150mm, 5ц)
Mobile Phase: Acetonitrile:Water:Acetic acid =20∶80:
流动相:乙***:水:醋酸=20∶80:(V/V/V) Flow Rate: 流速
Wave Length: 220nm 波长 Column: room temperature 测柱: 室温
solution 标准溶液
Transfer about 8 mg amygdalin standard(pany .), accurately weighed, to a 25mL volumetric flask, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water. Then add water to scale mark, it is obtained. 精确称量8mg(美国sigma公司)标准苦杏仁甙,放入 25ml 的量杯, 加适量水溶解,用超声波震动直至全溶。然后将水加至标度处,获得标准溶液。
solution 测试溶液
Transfer 8mg amygdalin powder, accurately weighed, to a 25ml volumetric flask
, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water, Then add water to scale mark, shake evenly, it is obtained.
精确称量8mg苦杏仁甙粉末,放入25ml的量杯中,加入适当水溶解,用超声波摇动直至溶解。然后加入水至标度处,均匀摇晃,获得测试溶液。
5. Procedure 过程
Separately inject equal volumes (about 20 uL) of the Standard preparation and the