文档介绍：该【分子生物学 】是由【小屁孩】上传分享，文档一共【49】页，该文档可以免费在线阅读，需要了解更多关于【分子生物学 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此文档到您的设备，方便您编辑和打印。Non-hethree-dimensionalstructureofmacromoleculesThebiologicalpropertiesofmacromoleculearemainlydeterminedbyactivatinginteractionsthatresultsineachmoleculeacquiringauniquethree-,andforanygiventypeofmacromolecule,severaloftheseweakerinteractionsmayplayaroleindeterminingthree-(分子内)interactions,eachmonomerwouldbefreetorotatewithrespecttoitsadjacentmonomersFew,ifany,(约束)alinearchaintofoldinsuchawaythatdifferentregionsofthechain,(二硫键;covalent):molecularweightChromatography层析:charge(positivelyornegativelycharged)oraffinitypropertiesElectrophoresis电泳:SDS-PAGE,chargeandsize;mostconvenientmethodUltracentrifugation超离心AminoacidsequencingX-raycrystallographyx射线晶体学Electronmicroscopy电子显微镜MacromoleculeisolationandcharacterizationNucleicacidGenecloningPCR:polymerasechainreactionMolecularsieves:molecularweightElectrophoresis:Agarosegel,mostconvenientmethodUltracentrifugationNucleotidesequencingElectronmicroscopySummaryponentsofmacromoleculesNoncovalentinteractionsCovalentbondsBondsfordeterminingthree-dimensionalstructureMethodsforisolationandcharacterizationofproteinandnucleicacidsImportantconceptsandaspectsyouarerequiredtounderstandSmallMoleculesvsMacromoleculesMonomers,monMonomersinCellsmon?ExceptionWhatmakeAAunique?DifferentcategoriesofAAandtheirspecialfeatures:formHbonds,saltbridges,disulfidebond,responsibleforUVabsorbance,etcDandLformsofAA4differentlevelsofproteinstructuresandtheirfeatures:basisofstructures,bondsinvolvedponentsofDNAandtheirpositionsindoublehelixstructureImportantforcesinmaintainingDNAstibilizationDifferencebetweenDNAandRNADandLsugarsharidesharidesharidesharides’majorrolesPeptidebondDisulfidebondglycosidicbondStructuralcharacteristicsoflipidsNoncovalentinteractionsMethodsforproteinisolationandcharacterizationMethodsfornucleicacidisolationandcharacterizationTheendofchapter2Chapter6 ,includingstorage,transmission,stability,.Avery/