文档介绍：中国农学通报 2011,27(05):475-478
Chinese Agricultural Science Bulletin
酶法提取马齿苋多糖
钱志伟 1,王会晓 2,杨海蛟 1
(1河南农业职业学院,郑州 451450;2郑州轻工业学院食品与生物工程学院,郑州 450002)
摘要:为了优化马齿苋多糖的提取工艺,将纤维素酶用于提取马齿苋多糖,在单因素实验的基础上,采
用 L (34)正交实验法对酶法提取马齿苋多糖的条件进行优化,考察酶用量、酶解温度、pH、酶解时间对马
9 ℃
齿苋多糖提取得率的影响。结果表明:%、酶解温度40 、pH 、酶解时
间 110 min。该条件下,马齿苋多糖得率达到 %。酶法提取纤维素多糖工艺简洁,能量消耗减少,是
一种提取马齿苋多糖的的适宜方法。
关键词:酶;提取;马齿苋;多糖
中图分类号: 文献标志码:B 论文编号:2010-2281
Enzyme Extraction of haride from Portulaca oleracea L.
Qian Zhiwei1, Wang Huixiao2, Yang Haijiao1
( , Zhengzhou 451450;
1Henan Vocational College of Agriculture
, Zhengzhou 450002)
2School of Food and Biological Engineering, Zhengzhou University of Light Industry
In order to extraction of haride from Portulaca oleracea L.. The optimum enzyme extraction
Abstract:
technology was optimized with L (34) orthogonal test. The influence of enzyme dosage,extraction temperature ,
9
pH and extraction time on yield of haride was studied. The optimum conditions were as follows: dosage
of cellulose %, temperature 40℃, time 110 min, pH and extraction yield were %. It indicated that
enzyme extraction was a suitable method for extraction haride from Portulaca oleracea L. for it was
simple and has shorter extraction time.
enzyme; extraction; Portulaca oleracea L.; haride
Key words:
0 引言 1 材料与方法
马齿苋(Portulaca olerace