文档介绍：中药制剂工程与技术
2013 年第 14 卷第 35 期
酶辅助提取菠萝皮色素的工艺研究
冯中秀,来丽丽
(浙江中医药大学生物工程学院,浙江杭州 310053)
摘要目的:考察影响酶提取菠萝皮色素的主要因素,并确定酶辅助提取菠萝皮色素的最佳工艺条件。方法:采用单因
素实验以色素浸提值为指标,考察了浸提时间、浸提温度、乙醇浓度、料液比对菠萝皮色素提取的影响,通过正交试验得
到最佳的提取工艺。结果:酶辅助提取菠萝皮色素的最佳工艺条件是:乙醇浓度 70%,提取温度 50 ℃,提取时间 80 min,
料液比 1∶50。结论:酶辅助提取法具有提取率高、速度快,溶剂用量少、安全、节能、设备简单等优点。本实验为优化菠萝
皮色素的提取工艺奠定了基础。
关键词酶辅助提取;菠萝皮色素;正交试验
中图分类号: 文献标识码:A 文章编号:1671-0258(2013)05-0037-03
Technology of Pigment Extracted From Pineapple Peel
by Enzyme Auxiliary Method
Feng Zhongxiu,Lai Lili
(College of Bioengineering,Zhejiang Chinese Medical University,Hangzhou Zhejiang 310053)
Abstract Objective:To discover main affecting factors and optimize extraction process of pineapple peel pigment by en-
zyme auxiliary method. Method:Single-factor experiment was adopted and content of pigment from pineapple peel was
taken as extraction time,extraction temperature,ethanol concentration and the ratio of solid to liquid were se-
lected as variable factors. Result:The optimum process conditions of pineapple peel pigment were as follows:70% ethanol
for 1∶50 ratio of solid to liquid in the process of extraction,extracting for 80 min at 50 ℃. Conclusion:The advantages of
enzyme auxiliary method are high extraction rate,fast speed,less solvent consumption,safety,energy saving,simple equip-
ment,etc. This experiment provide reference for optimi