文档介绍：Development of a biointegrated mandibular reconstruction device consisting of patible titanium fiber mesh scaffold
骨兼容钛纤维网支架组成的生物合成方法对下颌骨缺损重建的研究
Makoto Hirota , Takaki Shima Department of Dental Engineering, Tsurumi University School of Dental Medicine
Biomaterials, 28 September 2015,(IF=)
实验步骤
我们设计了亚微米薄涂层钛纤维网状支架重建即刻负载下颌骨节段性骨缺损,并在体内和体外评估其骨的兼容性。评估在含及不含HA 涂层支架中人成骨细胞的黏附、增殖及骨钙素的表达。在兔下颌骨形成10 毫米长节段性骨缺损,使用含及不含HA 涂层支架重建缺损,并分别在9 和 21 周观察,评估周围支架的骨形成及骨与支架连接的机械强度。
A cuboid with 10 mm square and 5 mm thick
The fiber diameter was 20 mm and the posotity was 87%. The mean pore size was 80-110 mm (average 95 mm). Deformation of the scaffold was slight when a mm-diameter and 7 mm-length dental implant was placed into it with 40 Ncm torque
For the animal study, a segmental bone defect 10 mm in length was created in rabbit mandibular bone ,followed by its reconstruction using a Ti fiber mesh scaffold
The scheme of the three-point bending test to the Ti fiber mesh scaffold Although the scaffold was gradually deformed by the vertical force, it remained unbroken by approximately 500 N
Its strength was not affected by a thin hydroxyapatite (HA) coating. Before and after HA coating of the scaffold . After coating, the block appeared black . However, the interior of the scaffold has hardly changed, indicating HA-coating has little effect on its three-dimensional micro-structure
(A) A