文档介绍：医学论文-PEP
              作者:丁鹏,王家宁,黄永章,骆丽娜,邵芳
【关键词】  ,超氧化物歧化酶
    Transduction of human Cu, Znsuperoxide dismutase mediated by an PEP1 peptide into endothelial cells of human umbilical vein
【Abstract】 AIM: To construct a prokaryotic expression system of pET15bPEP1SOD1, and to determine whether the expressed PEP1SOD1 fusion protein can be transduced into cells. METHODS: SOD1 cDNA was amplified by PCR using pBluescript II SKSOD1 as template, and digested with restriction endonuclease to construct binant plasmids pET15bSOD1 and pET15bPEP1SOD1, followed by being respectively transformed into to produce SOD1 and PEP1SOD1 fusion proteins, which were purified and added in cultured endothelial cells of human umbilical vein in vitro. RESULTS: The highly purified and active SOD1 and PEP1SOD1 fusion proteins were PEP1SOD1 fusion protein could enter endothelial cells of human umbilical vein in a time and dosedependent manner when added exogenously in a culture media. Once inside the cells, PEP1SOD1 protein was enzymatically stable for 48 h. CONCLUSION: PEP1SOD1 fusion protein can be transduced effectively into endothelial cells of human umbilical vein, which provides a basis for future study involving direct delivery of SOD1 protein into tissues for therapy.
【Keywords】 superoxide dismutase; prokaryotic expression; PEP1 peptide;  fusion protein
【摘要】目的:构建原核表达载体pET1