文档介绍：ABSTRACT
Fungal lignocellulose degrading enzymes genes could be induced by the soluble in. ducing molecules,and fungal cellulase gene expression is rolled by transcrip— tional actiVators and ,identification and characterization of transcrip- tional factors inVolVed in cellulase gene expression could be great useful for improVing the production of lignoceUul01)rtic enzymes.
In order to discoVer the noVel transcriptional genes involVed in cellulase gene expression,57 mutants of C2H2 zinc finger transcription f-actors family were screened with 2%crystalline cellulose as the sole carbon by measuring protein,endo-beta-l,4-glucanase actiVity,cellobiohydrolase actiVity,beta-glucosidase actiVity and to screening ,protein and endo—beta一1,4-glucanase actiVity were significantly increased by 25%-75%in 7 mutants,while 4c8口一』and 4cP口一2 were significantly reduced by 65%-80%
at protein and endo-beta-1,4一glucanase inVestigations in present studies focused on cP口一』and ce口一2 ,plemented transf-onnants were con— stmcted,锄d were fouIld phenotypically indistinguishable f-rom wild type ,the deficiency of△cP口一J and△cP以·2 in cellulase gene expression was indeed due to the disrl-lp- tion of cP口-J and as expected,these t、vo transcriptional flactors were to be
localized to the ,the transcriptome profiling analysis of△cP口一J and
△cP口-2 in response to AVicel showed that deletion of these two tmscriptional fIactors caused lots of ceUulase genes significantly down- other transcriptional fhc- tors inVoVled in lignocellulol”ic gene expression were also regulated by cP口一,and cP以一2, such as cellulase genes expression actiVators Clr·1,Clr-2 and carbon cat￡lbolite repressor
CRE--expression of cP口一J and cP口一2 would dramaticaUy improVe protein produc- tion a11d cellulase actiVity.
In conclusion,these findings suggest that these two noVel transcription f.