文档介绍：ABSTRACT
Objective: To obtain 8-(N,N-dimethyl-amine methyl)-baicalin by the chemical modification of baicalin, make a preliminary analysis of the structure and discuss the bacteriostasis of 8-(N,N-dimethyl-amine methyl)-baicalin, so as to expand the field of application of 8-(N,N-dimethyl-amine methyl)-baicalin.
Methods: 8-(N,N-dimethyl-amine methyl)-baicalin was prepared by Mannich reaction, its purity was detected by high performance liquid chromatography and a preliminary analysis of the structure was detected by mass spectrometric detection and carbon spectrum detection. Eight standard strains and two clinical isolated strains were selected as the tested strains to detect the minimal inhibitory concentrations
(MIC) 、 MIC50 、 MIC90 and the minimum bactericidal concentration (MBC) of
8-(N,N-dimethyl-amine methyl)-baicalin and baicalin. 8-(N,N-dimethyl-amine methyl)-baicalin were detected as the experimental group and baicalin were detected as the control group pare the similarities and differences of the antibacterial effect among the two different drugs on the same strains of bacteria. The clinical isolated strains were detected as the experimental group and the standard strains of the same bacteria were detected as the control group pare the similarities and differences of the antibacterial effect of 8-(N,N-dimethyl-amine methyl)-baicalin among the standard strains and clinical isolated strains of the same bacteria.
Results: The purity of 8-(N,N-dimethyl-amine methyl)-baicalin is % and the relative molecular mass of 8-(N,N-dimethyl-amine methyl)-baicalin is . 8-(N,N-dimethyl-amine methyl)-baicalin and baicalin were found to have certain bacteriostatic effects on the tested strains, but the bacteriostatic effects among different bacteria showed some differences. The MIC, MIC50, MIC90 and MBC of 8-(N,N-dimethyl-amine methyl)-baicalin were significantly lower than those of baicalin on the standard strains of Escherichia coli, Klebsiella pneumonia,